The MTT reagent was incubated with cells for 4 h. These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence polarization, and time-resolved. siRNA-mediated knockdown of KLF2 in RAW 264. Instruments and Settings for Fluorescence Read-out (Other Fluorescent Assays) FLUOstar Omega, VANTAstar, CLARIOstar, PHERAstar, PolarStar Optima. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. SpectraMaxi3,M3,M4,M5,M5e,andFlexStation 3instruments G03 through G08forSpectraMaxLinstrument Cross-talk D09 through D11,E09 through E11,andF09 through F11 for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. 2. 4 Cell viability assay The colorimetric 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was employed to determine cell viability; 1. User-installable and removable detection cartridges allow the detector to be configured for specific applications and easily. For more detailed information and technical support of Molecular Devices instruments or software, Luciferase Detection Instrumentation Guide. Walter2, and Roch-Philippe Charles1 1Institute for Biochemistry and Molecular. After 3 times washing, cells were added into 96-well plate and were examined under a fluorescence microplate reader (Excitation, 345 nm; Emission 495 nm) (SpectraMax M4 Multi-Mode Microplate Reader, Molecular Devices, San Jose, CA). Large molecule bioanalysis, immunogenicity and PK testing with higher quality data, rapid turnaround and fewer surprises. 315 can't find zero order. The samples were centrifuged to pellet the remaining intact erythrocytes, and supernatants were collected. , 2020b). Results were normalized and expressed as a percentage of live cells. Roelli1, Amandine Stoss1, Rebecca A. Test samples were measured according to the manufacturer’s instructions using a spectrophotometer SpectraMax M4 (Molecular Devices, Sunnyvale, CA). The available phosphorus in the soil was leached by double-acid extraction (0. of a 96 well-plate and cultured for 24h at 37 °C. Bioz Stars score: 98/100, based on 1 PubMed citations. Serving Victoria's renovation and construction needs. Also, when using a SpectraMax M4 Reader, the GxP audit trail incorrectly re ported the instrument as a SpectraMax M3 Reader. In luminescence mode, if you are specifying a wavelength for the read, you can select an emission bandwidth of either 15 nm or 25 nm. However, whether the Rhizoma drynariae total flavonoids (RDTF) can promote the absorption of calcium and enhance the bone formation is unclear. Lysosomal proteolytic activity. Serum C-reactive protein (CRP) was quantified using the Mouse C-Reactive Protein/CRP Quantikine ELISA Kit. These settings are also valid for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. Products : SpectraMax M3 reader, SpectraMax M4 reader, LMax II (Obsolete), FlexStation 3 reader, SpectraMax L reader, SpectraMax M5/M5e reader How do I know if the LM-1 plate is off or on? The LM1 plate has a push button switch in a cupped-out area on one end. 39. Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, Gemini EM reader, SpectraMax M5/M5e reader, FlexStation 3 reader, SpectraMax M4 reader Which is better: top-read or bottom-read? You can use either. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. The intestinal barrier has been recognized as being of major relevance for a number of diseases, namely digestive diseases like inflammatory bowel disease (IBD), irritable bowel syndrome (IBS), and celiac disease (CD), but also extra-intestinal diseases, such as asthma, arthritis, and the metabolic syndrome [1–4]. Kinetic fluorescence measurements were taken for 45 min at 37 °C in a SpectraMax M4 microplate reader (540 nm ex /590 nm em). Interpolated concentrations of native CRP in human serum and plasma samples. 2. Calpain activity assay The cells were treated with different drugs for 24 hours and were resuspended in 100 μL extraction buffer and homogenized by pipetting. Table 2. To determine the cell viability, thiazolyl blue tetrazolium bromide (MTT) assay was performed. VIEW DETAILS. Dettmer4, Wayne A. Measurements of temperature increase (ΔT) during the exposure of MNP/AMF hyperthermiaThe culture medium was removed, and the blue product was dissolved in DMSO (100 μl). item 7 replacement bulb for molecular devices spectramax m4 flash lamp replacement bulb for molecular devices spectramax m4 flash lamp. • SpectraMax® Paradigm (multi-mode) • SpectraMax® M5e / M5 (multi-mode) • SpectraMax® M4 / M3 (multi-mode) • SpectraMax® M2e / M2 (dual mode) • Gemini ™ EM and Gemini ™ XPS (fluorescence) • SpectraMax® L • SpectraMax® Plus384 (serial number # -MNR-xxxxx)The SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science. SpectraMax® ABS Plus. Instruments Ltd. Raw data generated on SpectraMax M4 Multi-Mode Microplate Reader is shown in the table. neoformans was cultured from glycerol stock in 5 mL of YM broth at 25°C for 72 hours at 200 rpm. item 3 replacement bulb for molecular devices spectramax xps xenon, spectramax xs xenon replacement bulb for molecular devices spectramax xps xenon, spectramax xs xenon. The absorbance was measured spectrophotometrically using a microplate reader (SpectraMax M4, Molecular Devices, CA, USA) at 570 nm. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port. For the SpectraMax M3/M4/M5 readers - use the Purple Assay Plate adapter for Top Read only. 05 mg mL −1. es • Flexibility for your assay needs: Robotics compatibility for increased throughput:すべてのSpectraMax® Mシリーズマルチモードマイクロプレートリーダーで、マイクロプレートおよびキュベットを使用して、紫外光・可視光吸光度ならびに蛍光強度を測定できます。. Prepare 22 mg/mL of FD4, 16 mg/mL of rhodamine70, and 40 mg/mL of creatinine in sterile PBS. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional capabilities allow you to upgrade with other detection modes at a later time. Samples (2 mg) were mixed with potassium bromide in a 1:100 ratio before the analysis. SpectraMax M4 Multi-Mode Microplate Reader. We have 2 Molecular Devices SpectraMax M4 manuals available for free PDF download: User Manual . data generated on SpectraMax M4 Multi-Mode Microplate Reader is shown in the table. trophotometer (SpectraMax M4, Molecular Devices, USA). Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. book Page 1 Wednesday, July 7, 2010 2:09 PM M4 SpectraMax M5 SpectraMax M5e Absorbance FluorescenceIntensity Luminescence Time-Resolved Fluorescence FluorescencePolarization ReadModes The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. Other technologies such as imaging. com. August 16, 2017. Hexokinase reagent (Eagle Diagnostics) was added and the reaction absorbance was read at 340 nm using a SpectraMax M4 multimode plate reader (Molecular Devices). Luminescence was immediately detected by the SpectraMax M4 Microplate/Cuvette Reader (Molecular Devices, San Jose, CA, USA). Firefly luciferase, a 61 kD monomeric protein, is especially attractive to many researchers because of its high sensitivity, wide linear detection range and extremely low background due to the absence of endogenous luminescent activity in mammalian cells. The SpectraMax M4 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96 and 384-well microplates. . The polysaccharide HPA with different concentrations (0, 25, 50, and 100 μg/mL) was added and incubated with cells for 24 h. What do I do now? In our tests, the Greiner Bio-One Fluotrac™ 200 Microplate has shown lower background. Record fluorescence emission data in kinetic mode for 2 min with 20 s between reads to ensure that interaction has reached equilibrium and take average. book Page 1 Wednesday, July 7, 2010 2:09 PM SpectraMax M4 reader had identical performance specifications to the SpectraMax M5 reader for absorbance and fluorescence detection. The microplate was exposed to an excitation wavelength of 530 nm (peak excitation is 540 nm), and the emission at 590 nm (peak emission is 580 nm) is recorded. Cet appareil ISM est confomre à la norme NMB-001 du Canada. All experiments were performed in triplicate. The SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science. 3. Compare plate readers7 Chapter 1: Installation and Setup SoftMax Pro Microplate Data Acquisition and Analysis Software controls Molecular Devices spectrophotometers, absorbance, luminescence, and fluorescence microplate readers, and the SpectraMax Paradigm Multi-Mode Detection Platform. Table 2. Ninety microliters of 2-chloro-4-nitrophenyl-α- d -maltotrioside substrate dilutions from 5000 to 1. Standard features include a cuvette port,. Also, when using a SpectraMax M4 Reader, the GxP audit trail incorrectly re ported the instrument as a SpectraMax M3 Reader. Fastenal is the largest fastener distributor in North America. Highly selective for dsDNA over ssDNA or RNA. MEK Inhibition Induces Therapeutic Iodine Uptake in a Murine Model of Anaplastic Thyroid Cancer Oussama ElMokh1, Vincent Taelman2, Piotr Radojewski2, Matthias A. free. Background-subtracted data values (mean +/- SD) are graphed. The absorption value at 570 nm was measured using a Molecular Devices SpectraMax M4 plate reader, and the absorbance at 690 nm was subtracted as the background. INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as wellThe SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading capability. Plot the fluorescence intensity versus the cell seeding number. Fluorescence intensities of each well were examined by a microplate reader (SpectraMax M4, Molecular Devices, CA, USA) with 485-nm excitation and 525-nm emission wavelengths. 5. Viability was determined by recording absorbance at 570 nm with plate reader (SpectraMax M4 Multimode, USA) and cell viability was determined. SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. Choose from a three- (M3), four- (M4), or We would like to show you a description here but the site won’t allow us. In this application note, we demonstrate the measurement of luciferase expression in CHO-K1 cells using the SpectraMax Glo Steady-Luc Reporter Assay Kit, which affords long-lasting luminescence signals. Note: In this user guide, SpectraMax ® refers to several SpectraMax . To report an issue with this product, Brief content visible, double tap to read full content. Measurement of Mitochondrial Membrane Potential by JC-1. 6 reviews. *Statistical significance: p < 0. Moving a SpectraMax Multi-Mode Microplate Reader. MTT assay was utilized to detect the cytotoxicity of different compounds on HUVECs. The absorbance of the biofilm was measured at 595 nm using a microplate reader (SpectraMax M4) and the results were expressed as percentage changes with respect to the control (without compounds). Then 10 µL of CCK-8 reagent was added to each well, and cell growth was measured at 490 nm using the SpectraMax M4 Multimode Microplate reader (Molecular Devices, San Jose, CA, USA). Statistical. For a complete list of the instruments supported by this release of the SoftMax Pro Software, see Supported Instruments on page 111. TEM. Raw data generated on SpectraMax M4 Multi-Mode Microplate Reader is shown in the table. . 75 × 10 5) suspended in PBS in 96-well dark plates incubated with NP or SiO 2 for 1 h at 37 °C. Example: [Drawer]. SpectraMax® L (software version 5. 2. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v2. SpectraMax 190: SpectraMax 340PC384: SpectraMax Plus384: VERSAmax: Vmax: Tecan: Sunrise: Trinean: DropSense96: AMBIENT TEMPERATURE STORAGE: Liconic: LPX 220 Hotel: LPX 440 Hotel: Thermo Fisher Scientific: Cytomat 10 Hotel: Cytomat 2 Hotel: Cytomat 24 Hotel: Cytomat 8 Linear Hotel: Cytomat 9 Hotel (MPH) BAR CODE READING: FluidX: Perception (Vial. Raw data generated on SpectraMax M4 Multi-Mode Microplate Reader is shown in the table. 005) Photometric PrecisionThe SpectraMax Paradigm Multi-Mode Detection Platform is a modular multi-mode microplate detector. /600 em. Plate chamber design of the SpectraMax category 1 microplate readers. Three kits covering a wide range of dsDNA concentrations. Choose from a three- (M3), four- (M4), orThe absorbance of the biofilm was measured at 595 nm using a microplate reader (SpectraMax M4) and the results were expressed as percentage changes with respect to the control (without compounds). After 3 times washing, cells were added into 96-well plate and were examined under a fluorescence microplate reader (Excitation, 345 nm; Emission 495 nm) (SpectraMax M4 Multi-Mode Microplate Reader, Molecular Devices, San Jose, CA). 7 cells. Dettmer4, Wayne A. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. 4. 22 μM was mixed with 10-μl enzyme giving a final enzyme concentration in the. ® reader to the computer. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Lastly, the TMB substrate (Beyotime) was added, and absorbance was measured at 450 nm by a microplate reader (SpectraMax M4). Results. After incubation, the biofilms were washed with PBS until fully remove crystal violet solutions and then, solubilized by adding a 150 μL acetic acid. WARNING! The instrument weighs approximately 36 pounds (16. Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, SpectraMax M4 reader, SoftMax Pro Software, SpectraMax Plus 384 reader, SpectraMax 190 reader, SpectraMax 340PC 384 (Obsolete), SpectraMax M5/M5e reader. Resolution: In the GxP audit trail, the SpectraMax M3 and M4 Readers are now identified correctly. Cell viability (%) was calculated as the ratio of the number of living cells in treated samples to that of the control [15,30]. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. The filtered samples were transferred to a 96-well plate and the fluorescence was measured using a plate reader (Spectramax ™ M4, Multi detection Reader) at excitation and emission wavelengths of λex 339 and λem 390 nm, respectively. book Page 1 Wednesday, July 7, 2010 2:09 PMTo quantify the cell density of bacterial cells, a SpectraMax M4 Microplate Reader was used to take measurements of the optical density at 600 nm. Page 17 Chapter 2: Set Up the StakMax Microplate Handler Microplate Handler Mounted Baseplate Connection This procedure provides instructions to connect the microplate handler to the following instruments: Gemini EM Gemini XPS SpectraMax M2 SpectraMax M2e SpectraMax M3 SpectraMax M4 SpectraMax M5 SpectraMax M5e SpectraMax. SpectraMax M3 Reader SpectraMax M4 Reader SpectraMax M5 Reader SpectraMax M5e Reader TRF,HTRF,FP HTRF, FP HTRF N/A Detection Modes Absorbance Fluorescence Intensity Luminescence Time-Resolved Fluorescence Fluorescence Polarization HTRF Upgrade Options Plate Formats 6-, 12-, 24-, 48-, 96-, 384-Well. 73. To assess the effect of CAD drugs on lysosomal activity in BMdM, fluorescence analysis using the DQ Green BSA probe was performed. Phillips5, Martin A. Analytical Technologies Group has deep experience maintaining and repairing Molecular Devices microplate readers, including the SpectraMax® M Series microplate readers (M2, M2e, M3, M5, M5, and M5e). The luciferase values were determined by normalization to Renilla values (as a control) to correct for variation in transfection efficiencies. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. The chamber contains springs that position the cuvette in the proper alignment for a read. While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of feature variability. 38. Members of the Sphingomonadales are renowned for their ability to degrade polycyclic aromatic hydrocarbons (PAHs). 5 mg mL −1 with 0. Examples: “Press carefully to engage,” “ Do not. Cleavage of the fluorescent NAG substrate (4-Methylumbelliferyl-2-acetamido-2-deoxy-β-D-Glucopyranoside) was measured for 20 min with 45 s intervals at 30°C in a Molecular Devices (San Jose, CA) SpectraMax M4 microplate reader. Large Molecule Bioanalysis. FilterMax F3 and F5 Multi-Mode; SpectraMax Paradigm® Multi-Mode. Then, MTT (10 μl of 5 mg/ml) was added to each well and further incubated for 4 h. Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, SpectraMax M4 reader, AquaMax washer, SpectraMax Plus 384 reader, SpectraMax 340PC 384 (Obsolete), SpectraMax L reader, SpectraMax M5/M5e reader. One of the. The following document is intended to demonstrate setup of this instrument. D 47 7. Microplate Readers include the M3, M4, exception is noted. Five hours prior to when permeability will be measured, gavage mice with 0. After incubating for 10 min at room temperature, the absorbances of standards and samples were measured at 450 and 590 nm, 12 using a Spectramax M4 spectrophotometer (Molecular Devices). 23, SpectraMax M3, M4, M5, and M5 e Multi-Mode Microplate Readers 0112-0115 F 23 PathCheck Pathlength Measurement Technology and Interfering Substances Any material that absorbs in the 900 nm to 1000 nm spectral region could interfere with PathCheck Pathlength Measurement Technology measurements. Detection modalities are shown in Table 1-1. StakMax Handling Stacker Base Unit. Eos - M4/3 lens adapter - Lenses - Victoria, British Columbia | Facebook. The use of gene reporters such as luciferase permits highly sensitive and nondestructive monitoring of gene expression. 1. These samples were analyzed using a multimode plate reader (SpectraMax M4, Molecular Devices, USA). The reaction systems were cultured in 37°C for 1 h, and the OD405 values were read on SpectraMax M4 (Molecular devices). The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). In luminescence mode, if you are specifying a wavelength for the read, you can select an emission bandwidth of either 15 nm or 25 nm. Molecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. • SpectraMax® M3/M4/M5/M5e Multi-Mode Microplate Readers • SpectraMax® Paradigm® Multi-Mode Microplate ReaderThe medium was replaced with CM from the macrophages of the different groups and then incubated for 48 or 72 h. Products : SpectraMax M2/M2e reader, SpectraMax M4 reader, SpectraMax M5/M5e reader, Gemini XPS reader, SpectraMax 190 reader, VersaMax reader, Gemini EM reader, SpectraMax Plus 384 reader. Indie Digital & Print Publishing. The ability of the liposome to be internalized into cell was assessed using cell culture studies 45-47. 2. 7°C (gradient heating design). Multi-Mode Microplate Readers including the M3, M4, M5, and M5. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional capabilities allow you to upgrade with other detection modes at a later time. For cell cultures, the medium was removed 24 h after initiation of drug stimulation and frozen for subsequent analysis, with no need for sample dilution. Dagda Lab. The IC50 curves were calculated using variable slope for log (inhibitor) vs. 6 Flexibility for your assay needs Robotics compatibility for increased throughput SpectraMax M Series readers can be easily integrated with our optional StakMax® Microplate Stacker forSpectraMax® M3. 5. Products : SpectraMax M4 reader, FilterMax F5 reader, SpectraMax Paradigm reader, SpectraMax M5/M5e reader Do you know any applications for TRF/HTRF that differ from Fluorescence Polarization? TR-FRET assays are well-suited for protein:protein interactions. Environmental Chamber and Respiratory SimulatorsRhizoma drynariae, a traditional Chinese herb, is commonly used in treatment of bone healing in osteoporotic fractures. We therefore gathered salient. The SpectraMax i3x reader has variable bandwidth for fluorescence and luminescence, and you are able to choose either 9nm or 15 nm for excitation and either 15 nm or 25 nm for emission. Find M4 Group Marketing Specialists in Victoria, with phone, website, address, opening hours and contact info. Lower limits of detection for ATP determined using the ATPlite . These settings are also valid for the SpectraMax M3/M4, FlexStation® 3 Multi-ModeThen, cells were stained with MitoSox Red (5 μM) for 30 min. The absorbance at 550 nm was recorded using a SpectraMax M4 microplate reader (Molecular Devices) following solvation of formazan with 100 μL DMSO. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. The plate reader optics are tunable for absorbance. Molecular Devices. SpectraMax® Paradigm® Multi-Mode Detection Platform. The microplate was exposed to an excitation wavelength of 530 nm (peak excitation is 540 nm), and the emission at 590 nm (peak emission is 580 nm) is recorded. Introduction. Colony formation assays were performed to measure cell proliferation. For problems with SpectraMax Multi-Mode Microplate Readers that are not listed here, in the U. com. Quick, Simple Standardization: The new analyzer features SPECTRO’s proprietary iCAL 2. In. Then the formazan crystals were solubilized in dimethyl sulfoxide, and the absorbance was recorded at 572 nm wavelength using a multimode plate reader (SpectraMax M4, Molecular Devices, USA). SpectraMax M Series Multi-Mode Microplate Readers | Molecular Devices · 2020-04-14 · use two scanning monochromators so the user can determine optimal excitation and emission settings, of 8 /8 Match case Limit results 1 per pageThe culture medium was removed, and the blue product was dissolved in DMSO (100 μl). ZERO BIAS - scores, article reviews, protocol conditions and more7. book Page 1 Wednesday, July 7, 2010 2:09 PM. The SpectraMax M2 and M2e systems provide the multi detection microplate readers with dualmonochromators, dual-mode cuvette ports, with top & bottom reading. The enzymatic reaction was triggered by addition of luminol and the chemiluminescence signal was monitored at 37 °C using a microplate reader SpectraMax M4 (Molecular Devices, Sunnyvale, CA, USA), with one acquisition every 2 s for 300 min. Category 2 and 3 microplate readers use gradient heating as well. Louis, MO, USA). FlexStation™ I. Plot the fluorescence intensity versus the cell seeding number. Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. Immunodetection Protocols for Cell Culture. The OD value of each well was determined using a SpectraMax M4 Multimode Microplate reader (Molecular Devices, USA), and the concentration of cytokines was calculated by comparing their OD value with the corresponding standard curve. 383 inches whereas the maximum allowed is 5. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v1. The SpectraMax®M3, M4, M5, and M5eMicroplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading capability. Background-subtracted data values (mean +/- SD) are graphed. Products : SpectraMax M3 reader, SpectraMax M4 reader, SpectraTest Validation Plates LM1 , FlexStation 3. MEK Inhibition Induces Therapeutic Iodine Uptake in a Murine Model of Anaplastic Thyroid Cancer Oussama ElMokh1, Vincent Taelman2, Piotr Radojewski2, Matthias A. We would like to show you a description here but the site won’t allow us. Molecular Devices SpectraMax ® M3/M4/M5/M5e Multi-Mode Microplate Readers + + Molecular Devices SpectraMax. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual- monochromator microplate reader platform offering a wide range of. Molecular Devices recommends that you use high-quality, doubleshielded cables to connect your SpectraMax. When the user applies a set temperature of 37°C, the top is heated to 37. Devices SpectraMax® M4). Cells were exposed to drug solutions with. Keywords: spectramax m2 systems, spectramax m2e systems, spectramax m2/m2e readers, pathcheck sensor, spectratest abs1 absorbance, stakmax stacker Created Date: 5/20/2019 9:24:38 AM SpectraMax M4 Multi-Mode Microplate Reader. AM (~0. NCI-60 cell panel screening was performed by the NCI/National Institutes of Health developmental therapeutics program (Bethesda, US). Specifications LUMINESCENCE PHOTOMETRIC PERFORMANCE Detection limit (384-well microplate) 10 amol/well alkaline phosphatase, 200 µL Wavelength range 250–850 nm TIME-RESOLVED FLUORESCENCE PERFORMANCE SpectraMax M3 reader SpectraMax M4 reader SpectraMax M5 reader SpectraMax M5e reader Detection modes Absorbance ü ü ü ü Fluorescence intensity ü ü ü ü Luminescence ü ü ü ü Time-resolved fluorescence ü ü ü Fluorescence polarization ü ü HTRF ü Upgrade options TRF, HTRF, FP HTRF, FP HTRF N/A Plate formats The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Because of possible losses from settling, particle data for the >7 µm size fraction (<0. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™ Multi-Mode Detection Platform. org) with optimized instrument settings (Table 1) were used to generate and analyze data. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening. For the FlexStation 3 Reader - Keep the Black Plate Adapter in place fro both Top and Bottom reads. For more detailed information and technical support of Molecular Devices instruments or software,The sample solution was added to DPPH. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. However, dynamic changes in soil microbiota under different tree ages have received little attention. ” The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Optimized for fluorescence microplate readers. Table 2. 9000-0756. Plates were then read at 450 nm using a SpectraMax M4 device (Molecular Devices, Sunnyvale, CA). 2. SpectraMax Gemini, SpectraMax I series, SpectraMax M series, FlexStation 3. Multi-Mode Microplate Readers including the M3, M4, M5, and M5. GSA System Includes: SpectraMax M4 Multi-Mode Spectrophotometer with Tunable. Background-subtracted data values (mean +/- SD) are graphed. 2. org) with optimized instrument settings (Table 1) were used to generate and analyze data. If you need to relocate a SpectraMax Multi-Mode Microplate Reader, follow these steps. "With the launch of the Molecular Devices® SpectraMax® M3 and M4 Multi-Mode Microplate Readers, we now offer a full range of multi-mode detection capabilities within our SpectraMax® product line," said Andy Boorn, President of MDS Analytical Technologies. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Z’-LYTE® Assay using the Z'-LYTE® Tyr6 kit. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. 1. SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating. Description 1. Figure 1. Background-subtracted data values (mean +/- SD) are graphed. The optical density at 450 nm was detected using a microplate reader (SpectraMax M4, MolecularDevices, CA, USA). RESEARCH PAPER Effect of particle size and surface charge of nanoparticles in penetration through intestinal mucus barrier Sony Priyanka Bandi & Yashada Sanjay Kumbhar & Venkata Vamsi Krishna VenugantiThe Fibrinogen standard curve was prepared as described in Section 10. 8. , UK) or SpectraMax M4 by Molecular Device. Establishment of mouse orthotopic xenograft bladder tumor model. Calpain activity assay The cells were treated with different drugs for 24 hours and were resuspended in 100 μL extraction buffer and homogenized by pipetting. Spectramax M4 Multi mode Microplate Detection System: Benchpro 4100 instrument card processing station: Muse Cell Analyzer: Veriti 96w Thermal Cycler: Small Animal Anaesthesia System: HPLCs (Analytical & Preparative) ACQUITY UPLC H-Class Bio: LC-MS/MS Q-tof 6540: Gas Chromatograph: GC-2014: Automatic Digital Polarimeter: FT-IR. Raw data generated on SpectraMax M4 Multi-Mode Microplate Reader is shown in the table. spr SpectraMax iD3 SpectraTest ABS2 v1. SpectraMax® M4 Multi-Mode Microplate Reader MLDVM4Relative florescence units (RFU) were quantified on a plate reader (SpectraMax®; M4; Molecular Devices, Sunnyvale, CA) every 1 minute over a 10‐minute period at 37°C, using excitation and emission wavelengths of 328 and 420 nM, respectively. DNA standards. FD-4 concentrations in plasma were analyzed in duplicates using a spectrophotometer (SpectraMax M4, Molecular Devices, San Jose, CA, USA) with excitation λ 485 nm and emission λ 535 nm. STAKMAX. Consult Molecular Devices's entire SpectraMax M series catalogue on MedicalExpo. 1 M Tris solution and the fluorescence of the solution was measured using a fluorometer (SpectraMax M4, Molecular Devices). Steady-state kinetics measurements were performed at 25°C in 96-well plates (Corning) using a SpectraMax M4 spectrophotometer (Molecular Devices) in PBS buffer. For more detailed information and technical support of Molecular Devices instruments or software,Luciferase Detection Instrumentation Guide. Optimized for fluorescence microplate readers. SpectraMax® M4). F1 SpectraMax Plus384andABSPlus: Certificate Value – 2≤ Average Peak Value≤ Certificate Value + 2 SpectraMax M2,M2e,M3,M4,M5,andM5e: Certificate Value – 3≤ Average Peak Value≤ Certificate Value + 3 Photometric Accuracy F2, F3, F4 AverageOD=Certificate Value± (Certificate Value(0. Assessment of Neurite Outgrowth. SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. The absorption value at 570 nm was measured using a Molecular Devices SpectraMax M4 plate reader, and the absorbance at 690 nm was subtracted as the background. 01) + 0. (Shanghai, China). 発光モードに対応した機種（SpectraMax M5e/M5/M4/M3）では、発光モード専用のフォトンカウント検出器も搭載しました。 蛍光に特化した検出器を流用するのではなく、専用の光学系を採用することで、発光、TR-FRET、蛍光偏光アッセイにおいても専用機に匹敵す. For SpectraMax L or LMax II, LMax II 384 - no adapter used. 305 reference level saturation. The whole process was protected from direct light. alamarBlue reagent volume in a different spheroid microplate formatA SpectraMax M4 microplate reader (Molecular Devices, LLC, Sunnyvale, CA, USA) was used to measure the absorbance at a wavelength of 450 nm. SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. The Insulin standard curve was prepared as described in Section 10. The UV‐Vis absorption spectra and cell viability were measured by a multi‐mode microplate reader (SpectraMax® M4, Molecular Devices LLC) using quartz cuvettes with 1‐cm path length (Starna Scientific Ltd. 6 mm). 1step assay with SpectraMax microplate readers. 161 likes. At sacrifice, serum was isolated from mouse total blood and maintained at –80 °C. † Bottom mounting for SpectraMax Paradigm Readers only. At the end of the incubation period, MTT was added and the plates were incubated for 4 h at 37°C. Microplate Reader Shelf. ® reader to the computer. for Cisbio Bioassays. The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quantitation of firefly luciferase expression in mammalian cells. When you flip the LM1 plate over for checking Bottom Read, remove the Purple Plate adapter. The medium was then removed and 100 μl of DMSO was added to dissolve the formazan crystal. SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. The microplate was exposed to an excitation wavelength of 530 nm (peak excitation is 540 nm), and the emission at 590 nm (peak emission is 580 nm) is recorded. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. 0. All experiments. Fifteen different automated tests are provided: • Background noise • Background spike • Lower limit of detection • Crosstalk • Linearity • Relative luminescence units (RLU) • Kinetic noise (low signal) 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuide SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. spectramax multi-mode microplate Readers The SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. 2. Table 1. 2. Other technologies such as imaging. 9000-0133 SpectraMax®MousePad 9000-0972 Packageof5samplemicroplates 2400-2464 DustCover 5000505 CEDeclarationofConformity 5019680 Card,UserGuideDownload The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. How do I set up Hellma Cuvette validation set recertification? Send Hellma cuvette standards to Hellma. 5 mg mL −1 with 0. , San Jose, CA) was. After incubation at 37 °C for 3 h, the fluorometric detection of cleaved AFC and chromogenic product of pNA was performed on a Multimode Plate Reader (Spectramax M4, Molecular devices, USA) using a 400 nm excitation filter and a 530 nm emission filter for detection of caspase 3 and 410 nm wavelength for detection of caspase 9 using. 0 compensates for most changes in environmental temperature or pressure. The SpectraMax M4 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96 and 384. 5 mL of creatinine, FD-4, and rhodamine70 dissolved in. ATP standard curves read on SpectraMax microplate readers. . Results were expressed as a percentage relative to the control group (defined as 100%). Tris solution and the fluorescence of the solution was measured using a fluorometer (SpectraMax M4, Molecular Devices). While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of. 95. SpectraMax® M4). 116 SpectraMax M5 and M5e Multi-Mode. 4. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Keywords: spectramax m2 systems, spectramax m2e systems, spectramax m2/m2e readers, pathcheck sensor, spectratest abs1 absorbance, stakmax stacker Created Date:. D 47 7. The optical density was measured using a microplate reader (Spectramax M4, Molecular Devices, San Jose, CA, USA) at 570 nm. The chloride and pH. For SpectraMax L or LMax II, LMax II. AquaMax® 4000. . Absorbance was read at 570 nm with SpectraMax M4 microplate spectrophotometer (Molecular Devices, CA). In the competitive field of drug development where timely go/no-go decisions must be made to bring effective class-leading drugs to market, it is vital to work with a CRO that can provide highly. After 48 h, the optical density (OD) was measured at 450 nm using the microplate reader (SpectraMax M4). DNA standards ranging from 50 pg/mL to 1 μg/mL were detected using the Quant-iT PicoGreen dsDNA Assay Kit and SpectraMax readers (data from the SpectraMax iD5 reader are shown, but other SpectraMax readers gave similar results). com. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). The Dagda Lab is a mitochondrial biology-oriented research group located at the University of Nevada, Reno School of Medicine that currently spearheads basic and translational research projects supported. To investigate the pharmacokinetics of HSA–CBT-NPs, 10 male Sprague-Dawley rats (mean±SD=250±20A SpectraMax M4 microplate reader was purchased from MeiGu Molecular Co. At 48 h after transfection, the Nano-Glo Live Cell Assay reagent was added and the luciferase activity in the medium and cells were measured using a SpectraMax M4 (Molecular Devices, San Jose, CA, USA). SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. Choose from a three- (M3), four- (M4), orThe integration time suggested is 5-10 sec. The MDD was determined by calculating the mean of zero standard replicates (n=24) and. ITEM#: M4-MOL Molecular Devices SpectraMax M4 upgradable microplate/cuvette reader (90V to 240V) with current version of SoftMax Pro. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, SpectraMax M4 reader, SpectraMax M3 reader MTT reduction - a tetrazolium-based colorimetric assay for cell survival and proliferation (Maxline Application Note #5) (PDF) Summary: The MTT assay measures cell respiration and the amount of formazan. 50 μl of samples were added into the mixture of 40 μl of detection buffer and 10 μl of Ac-DEVD-pNA (2 mM) with gentle shaking for 5 min. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Plus 384 ABS1 Location: Reader Validation-Plate Abs A reminder about recertification was added. The absorbance was determined by a microplate reader (SpectraMax M4, USA) at 405 nm. Transfected cells were seeded into 6-well plates at a density of 200 cells/well. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax. 9000-0756. Supplier: Molecular Devices. Fluorescence Microplate Reader Comparison Chart. If using the StakMax and a SpectraMax reader together, the time specifications for the movement of the. Dissolve FITC-dextran in PBS at a. 次いで、ユーザーのラボに. : 915 515 403 • Fax: 914 334 545 • e-mail: info@bionova. Western blot apparatus (BIO-RAD Laboratories). Fluoresbrite® fluorescent nanoparticles were analyzed by spectrofluorimetry where particles were excited at 441 nm wavelengths and emission intensity was recorded at 486 nm wavelengths. overnight. MTT was then removed, and the formed formazan crystals were then dissolved in 150‐μl DMSO. Graph pad prism 8. Data are presented as mean values ± standard.